Takara T-Vector pMD20 \1 ug

Takara T-Vector pMD20 is a linearized vector with a single 3-terminal thymidine at both ends. The T-overhang ends at the cloning site improves the efficiency of ligation of PCR products which contain A-overhangs at 3-ends. T-Vector pMD20 is derived from a pUC19 backbone with modifications in the coding sequence to reduce the false positive rate and improve the positive colonies screened by blue/white color on ampicillin/IPTG/X-Gal LB plates. In addition, an SP6 promoter has been inserted upstream of the multiple cloning site. Therefore, the inserted DNA fragments can be transcribed by SP6 RNA Polymerase (Cat. #2520). T-Vector pMD20 is supplied with a Control Insert (500 bp) DNA for positive control reactions. - TAKBGN (Additional S&H or Hazmat Fees May Apply)