Revvity Nextflex Poly(A) Beads 2.0

• 10 ng – 5 µg total starting RNA
• Magnetic bead-based protocol
• No organic solvents or precipitation step required
• Tested using the NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
• Optimized for use with 10 ng – 5 µg of total RNA as starting material
• Automated on the Sciclone^® G3 NGSx and Zephyr^® G3 NGS workstations

Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes, thereby eliminating the need for precipitation. These beads have been validated with the new and improved NEXTFLEX^® Rapid Directional RNA-Seq Kit 2.0.