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Fluorescence detection is a direct Western blotting method in which a secondary antibody is conjugated to a fluorophore, avoiding the need for ancillary detection reagents.
Amersham Cy3 and Cy5 secondary antibodies are labeled with near-infrared (NIR) fluorophores that emit light at wavelengths of 570 and 670 nm. By using Amersham Cy3 and Cy5 secondary antibodies together with a pair of appropriate rabbit or mouse primary antibodies, you can perform multiplexed experiments.
Key benefits:
Broad dynamic range
High sensitivity
Ability to detect multiple proteins on same blot (multiplexing)
Wide pH tolerance (3 to 10), making them compatible with most Western blotting buffers