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Alkaline phosphatase is suitable for dephosphorylation of 5 and 3-overhangs, recessed ends, blunt ends, and single nucleotides. Following incubation, alkaline phosphatase can be easily inactivated by heating.
Bacterial alkaline phosphatase recombinantly expressed in E. coli.
Active in PCR and restriction endonuclease buffers.
Inactivated by heating at 75°C for 5 minutes.
Use together with Exonuclease I for cleaning up PCR reactions prior to downstream application such as DNA sequencing.
Unit Definition
One unit is the amount of the enzyme required to dephosphorylate 1 µg of linearized pUC57 DNA termini in 10 minutes at 37°C.
