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Cytiva Superdex 30 Increase SEC columns are for high resolution, small-scale purification and analysis of small biomolecules with Mr ~ 100 to ~ 7000, such as peptides
Superdex 30 Increase SEC columns are for high resolution, small-scale purification and analysis of small biomolecules with Mr ~ 100 to ~ 7000, such as peptides
Improved performance – improved resolution and runtime compared to predecessor Superdex Peptide
Tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover.
Very high resolution – small bead size and narrow particle size distribution provide high resolution, for high protein purity.
High flow rate – due to rigid beads giving excellent pressure/flow properties.
New generation of size exclusion chromatography columns
Superdex 30 Increase is part of Cytivas new generation of agarose-based SEC columns called “Increase”, which offer higher resolution purification in shorter runtimes compared with their predecessors.
SEC columns for analysis and purification of peptides
Superdex 30 Increase is suitable for both preparative and analytical purposes.
The fractionation range allows preparative separation of peptides, oligosaccharides and other small biomolecules (Mr ~ 100 to ~ 7000) with sample volumes up to 500 µL.
These columns also enable protein characterization, including aggregate analysis, size homogeneity analysis, and interaction studies, where high-quality data can be achieved
Cleaning-in-place (CIP) capabilities
This agarose-based resin is alkali resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different biomolecules, with minimal risk of carry-over between samples.
Regular cleaning can also increase column lifetime.
Two SEC column sizes
Two size exclusion chromatography (SEC) column sizes are designed to serve different purposes:
10/300 GL column, 10 mm × 300 mm, for high resolution analysis as well as for small-scale (25–500 µL) preparative purification, such as the polishing step in a purification protocol
3.2/300 column, 3.2 mm × 300 mm, for high resolution analysis and purification of sample volumes of 4–50 µl, when sample amount is limited and low buffer consumption is important
It is also possible to order Superdex 30 Increase resin to be packed in 5/150 GL column, 5 mm x 150 mm, as custom designed product.
Other Cytiva size exclusion chromatography columns
Cytiva offers a wide range of Life Sciences solutions for SEC (or gel filtration). Let Cytiva help you find the right SEC column for your needs.
