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Agilent Technologies Gk80040 Advancebio Sialidase A Sampler Kit, 200 Ul
List Price $353.00 Your Price $353.00
Agilent Technologies AdvanceBio Sialidase A, 1 U. Enzyme releases α(2,3)-, α(2,6)-, α(2,8)-, α(2,9)-linked N-acetylneuraminic acid from oligosaccharides and glycoproteins. Includes 5x Reaction Buffer B - BC (Additional S&H or Hazmat Fees May Apply)
GROUP SKU: GK80040
| Concentration | 5 U/mL |
| Enzyme Formulation | 20 mm Tris HCl pH 7.5, containing 25 mm NaCl. |
| Enzyme Source | Recombinant gene from Arthrobacter ureafaciens, expressed in E. coli. Sialidases are also known as neuraminidases. |
| Enzyme Specific Activity | 40 units/mg |
| Enzyme Specificity | The enzyme releases a(2,3)-, a(2,6)-, a(2,8)-, and a(2,9)-linked N-acetylneuraminic acid from complex carbohydrates. The initial rate of hydrolysis of a(2,6) linkages is reported to be approximately twice that of a(2,3)-linked sialic acid however, in practice, this kinetic selectivity is of little consequence during extended incubations. Effective digestion of glycolipid substrates is facilitated by addition of a detergent, such as sodium taurodeoxycholate to the incubation. Sialidase A is capable of releasing N-glycolylneuraminic acid (Neu5Gc, NGNA) in addition to N-acetylneuraminic acid (Neu5Ac, NANA) [1], although similarly to other sialidases [2] the activity is lower toward Neu5Gc than Neu5Ac. The enzyme can also be used to remove sialic acid from gangliosides [3], glycosphingolipids (ceramide and oligosaccharide) with sialic acid. May be used for exoglycosidase sequencing with Sialidase S (GK80021), which is specific for a(2,3)-linked sialic acids. Sialidases are also known as neuraminidase. |
| Enzyme Unit Definition | One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37° C, pH 5.5. |
| Molecular Weight | ~88 kD |
| Unit | 1 U |
| Volume | 200 µL |
| pH Optimum | 6 |
| SKU | GK80040 |
|---|---|
| Supplier Part Number | GK80040 |
| UM | EA |
| UNSPSC | 41116133 |
| Manufacturer | Agilent Technologies |
| MSDS URL | Click here |
| Refrigerated-Frozen | N |
| CountryOfOrigin | United States |
| ProductLine | BC |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |