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Agilent Technologies Gke-5003 Pngase F, 50 Ul
List Price $365.00 Your Price $365.00
Agilent Technologies PNGase F, Chryseobacterium, native. Native PNGase F, peptide-N-glycosidase F. Releases intact N-glycans by cleaving between innermost GlcNAc and Asn - BC (Additional S&H or Hazmat Fees May Apply)
GROUP SKU: GKE-5003
| Application | Structural analysis of O-glycan |
| Concentration | ≥2 U/mL |
| Enzyme Applications | PNGaseF is used to release intact N-linked glycans from glycopeptides and glycoproteins preparation of deglycosylated proteins for molecular weight estimation or crystallography studies structure-function studies of N-glycosylated glycoproteins |
| Enzyme Formulation | 52 mm Tris-HCl, 36 mm NaCl, 9 mm sodium acetate, 5 mm EDTA, 0.02% sodium azide (pH 7.5), glycerol-free formulation |
| Enzyme Source | Native, purified from Elizabethkingia meningoseptica. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum |
| Enzyme Specificity | PNGase F releases intact N-linked oligosaccharides from glycoproteins and glycopeptides. The enzyme will not cleave oligosaccharides from a single asparagine residue and cleavage does not occur if there is core α1-3 linked fucose as commonly encountered in plant glycoproteins Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. |
| Enzyme Unit Definition | One unit of N-Glycanase is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at pH 7.5 and 37°C |
| Unit | 100 mU |
| Volume | 50 µL |
| pH Optimum | 7.5 |
| SKU | GKE-5003 |
|---|---|
| Supplier Part Number | GKE-5003 |
| UM | EA |
| UNSPSC | 41116133 |
| Manufacturer | Agilent Technologies |
| MSDS URL | Click here |
| Refrigerated-Frozen | N |
| CountryOfOrigin | United States |
| ProductLine | BC |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |