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Agilent Technologies Gke-5006a-05 Advancebio N-Glycanase (Pngase F), 40 Ul
List Price $1,576.00 Your Price $1,576.00
Agilent Technologies AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL. Enzyme releases intact N-glycans by cleaving innermost GlcNAc and Asn. Includes 5 N-glycanase reaction buffer, denaturation solution, - BC (Additional S&H or Hazmat Fees May Apply)
GROUP SKU: GKE-5006A-05
| Concentration | ≥2.5 U/mL |
| Enzyme Applications | Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies Release of intact N-linked glycans from glycopeptides and glycoproteins Structure-function studies of N-glycosylated glycoproteins |
| Enzyme Formulation | 20 mm Tris-HCl, 50 mm NaCl, 1 mm EDTA (pH 7.5) |
| Enzyme Source | Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum [1]. Enzyme is also known asPNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. |
| Enzyme Specificity | Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless α(1-3) core fucosylated, as in plant glycans and some insect glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. This product is Endo F free. |
| Enzyme Unit Definition | One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at 37°C, pH 7.5. |
| Molecular Weight | ~35,000 daltons |
| Unit | 500 mU |
| Volume | 40 µL |
| pH Optimum | 8.6 |
| pH Range | 7.5-9.5 |
| SKU | GKE-5006A-05 |
|---|---|
| Supplier Part Number | GKE-5006A-05 |
| UM | EA |
| UNSPSC | 41116133 |
| Manufacturer | Agilent Technologies |
| Refrigerated-Frozen | N |
| CountryOfOrigin | United States |
| ProductLine | BC |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |