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Agilent Technologies Gke-5008 Advancebio Endo F2, 60 Ul
List Price $353.00 Your Price $353.00
Agilent Technologies AdvanceBio Endo F2, recombinant. Enzyme cleaves chitobiose (GlcNAc-GlcNAc) core of high mannose and biantennary complex N-glycans - BC (Additional S&H or Hazmat Fees May Apply)
GROUP SKU: GKE-5008
| Enzyme Applications | Selective release of high mannose and biantennary complex type N-linked oligosaccharides from glycopeptides/ glycoproteins. |
| Enzyme Formulation | 10 mm sodium acetate pH 4.5, 25 mm NaCl, 0.02% sodium azide |
| Enzyme Source | Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. |
| Enzyme Specificity | The enzyme cleaves within the di-N-acetyl chitobiose (GlcNAc-GlcNAc) core of high mannose and biantennary complex type N-linked glycans of oligosaccharide, glycopeptide, or glycoprotein substrates. A single GlcNAc residue remains attached to the molecule after hydrolysis, the rest of the glycan is released. This specificity is distinct from PNGase F (GKE-5006), which cleaves between asparagine and the innermost GlcNAc of N-linked glycans. EndoF2 cleaves between GlcNAc-GlcNac in biantennary glycans at a rate approximately 20 times higher than high mannose glycans. The enzyme is not active towards hybrid type or tri- and tetraantennary oligosaccharides. |
| Enzyme Unit Definition | One milliunit is defined as the amount of enzyme that will hydrolyze 1 nM of the dansylated porcine fibrinogen glycopeptide substrate DnsVGEN(CHO)R per minute at pH 4.5 and 37°C. |
| Unit | 6 mU |
| Volume | 60 µL |
| pH Optimum | 4.5 |
| SKU | GKE-5008 |
|---|---|
| Supplier Part Number | GKE-5008 |
| UM | EA |
| UNSPSC | 41116133 |
| Manufacturer | Agilent Technologies |
| MSDS URL | Click here |
| Refrigerated-Frozen | N |
| CountryOfOrigin | United States |
| ProductLine | BC |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |