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Agilent Technologies Advancebio Pngase A (Almond), 10 Mu
List Price $1,273.00 Your Price $1,273.00
Agilent Technologies AdvanceBio PNGase A (almond), 10mU. Enzyme cleaves N-linked glycans from glycopeptides. Includes reaction buffer - BC (Additional S&H or Hazmat Fees May Apply)
GROUP SKU: GKE-5011B
| Enzyme Formulation | Lyophilized powder containing citrate-phosphate buffer, pH 5.0. |
| Enzyme Source | Almond. Enzyme also knows as Peptide N-Glycosidase A, Glycopeptidase from almonds, Almond glycoamidase, Peptide-N(4)-(N-acetyl-β glucosaminyl) asparagine amidase. |
| Enzyme Specific Activity | > 5 units/mg |
| Enzyme Specificity | PNGase A from almonds cleaves N-glycan chains linked to Asparagine from glycopeptides. The enzyme hydrolyzes an N(4)-(acetyl-ß D-glucosaminyl) asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-ß D-glucosaminylamine and a peptide containing an aspartate residue. The enzyme cleaves N-glycans with or without a(1,3)-linked core fucose residues present in insect and plant glycoproteins. This differs from PNGase F, which cannot remove N-glycans with a(1,3)-linked core fucose. PNGase A requires glycopeptides as substrates. Glycopeptides can be obtained by digesting the glycoprotein with trypsin [1,2] or pepsin. Does not act on (GlcNAc)-Asn, because it requires the presence of more than two amino acid residues in the substrate. |
| Enzyme Unit Definition | One unit is defined as the amount of enzyme required to hydrolyze 1 µmol ovalbumin glycopeptide per min at pH 5.0 and 37°C. |
| Molecular Weight | 54.2 and 21.2 kDa |
| Unit | 10 mU |
| pH Optimum | 5 |
| pH Range | 4-6 |
| SKU | GKE-5011B |
|---|---|
| Supplier Part Number | GKE-5011B |
| UM | EA |
| UNSPSC | 41116133 |
| Manufacturer | Agilent Technologies |
| MSDS URL | Click here |
| Refrigerated-Frozen | N |
| CountryOfOrigin | United States |
| ProductLine | BC |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |