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Agilent Technologies Gke-5016b Advancebio N-Glycanase (Pngase F), 80 Ul
List Price $629.00 Your Price $629.00
Agilent Technologies AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL. Recombinant PNGase F (200 mU, EDTA free). Releases intact N-glycans by cleaving between innermost GlcNAc and Asn. Includes - BC
GROUP SKU: GKE-5016B
| Concentration | 2.5 U/mL |
| Enzyme Formulation | 20 mm Tris-HCl, 50 mm NaCl (pH 7.5) |
| Enzyme Source | Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. |
| Enzyme Specific Activity | ≥10 units/mg |
| Enzyme Specificity | Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions. |
| Enzyme Unit Definition | One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5. |
| Molecular Weight | ~35,000 Daltons |
| Unit | 200 mU |
| Volume | 80 µL |
| pH Optimum | 8.6 |
| pH Range | 7.5-9.5 |
| SKU | GKE-5016B |
|---|---|
| Supplier Part Number | GKE-5016B |
| UM | EA |
| UNSPSC | 41116133 |
| Manufacturer | Agilent Technologies |
| MSDS URL | Click here |
| Refrigerated-Frozen | N |
| CountryOfOrigin | United States |
| ProductLine | BC |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |