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Agilent Technologies a(1-2,3,6)-Mannosidase (Jack Bean)
List Price $965.00 Your Price $965.00
Agilent Technologies α(1-2,3,6)-Mannosidase, 50U, ≥150U/mL. Releases nonreducing terminal α(1-2,3,6)-linked mannose from oligosaccharides. Includes incubation buffer. - BC (Additional S&H or Hazmat Fees May Apply)
GROUP SKU: GKX-5010-50
| Concentration | 150 U/mL |
| Enzyme Formulation | 20 mm Tris-HCl, 20 mm NaCl (pH 7.5) |
| Enzyme Source | Jack bean. |
| Enzyme Specificity | The enzyme has broad specificity, cleaving a(1-2,3,6)-linked mannose, although some kinetic preference has been observed (a1-2, 6 > 3). The enzyme will not cleave a single a(1-6) linked mannose residue from core ß-mannose, but will, however, remove a single a1-3 linked mannose from the core ß-mannose. By using enzyme concentrations of around 50 U/mL and extended incubation times (up to 18 hours) at 37°C, complete removal of all nonreducing terminal a-linked mannose residues may be achieved. To expedite glycan sequencing studies, the sluggish activity of GKX-5010 jack bean a mannosidase toward a(1-6)-linked mannose residues can be overcome by using the enzyme in combination with the a mannosidase from Xanthomonas mannihotis that rapidly cleaves a(1-6) linkages. The X. mannihotis a-mannosidase may inhibit the action of other mannosidases if a branched (a1-6) mannose is present in the substrate, SO is typically added after incubation of the substrate with jack bean a-mannosidase (GKX-5010). |
| Enzyme Unit Definition | One unit is defined as the amount of enzyme that will hydrolyze 1 µmole of pNP-α-mannopyranoside per minute at pH 4.5 and 37°C. |
| Molecular Weight | ~190 kD |
| Unit | 50 U |
| Volume | 70 µL |
| pH Optimum | 4.5 |
| SKU | GKX-5010-50 |
|---|---|
| Supplier Part Number | GKX-5010-50 |
| UM | EA |
| UNSPSC | 41116133 |
| Manufacturer | Agilent Technologies |
| Refrigerated-Frozen | N |
| CountryOfOrigin | United States |
| ProductLine | BC |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |