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GenScript Bevacizumab Pharmacokinetic Elisa Kit; 96Tests
List Price
$498.00
Your Price
$498.00
GenScript Bevacizumab Pharmacokinetic Elisa Kit - GSCRPT (Additional S&H or Hazmat Fees May Apply)
NETA PART:
GSCRPT-L00969
MFG.PART:
L00969
UNSPSC:
41116126
Manufacturer:
GenScript USA Inc
Overview
| Product Description | Bevacizumab, with the brand name Avastin, is a humanized monoclonal antibody of IgG1 isotype. It targets the vascular endothelial growth factor A (VEGF-A), preventing its activation of VEGF receptor and inhibiting malignant cell growth and blood vessel formation. Bevacizumab is approved for the treatment of patients with cervical cancer, metastatic colorectal cancer, primary peritoneal cancer and so on. |
Product Introduction
| LLOQ | 39.06 ng/mL | ||||||||||||||||||||||||||||||
| ULOQ | 2500 ng/mL | ||||||||||||||||||||||||||||||
| Precision | Intra-assay: CV≤10% Inter-assay: CV≤15% | ||||||||||||||||||||||||||||||
| Minimum required dilution (MRD) | 1:50 selected by cynomolgus monkey plasma | ||||||||||||||||||||||||||||||
| Specificity | No cross-reactivity at 25,000 ng/mL of Human IgG1 | ||||||||||||||||||||||||||||||
| Hook Effect | Not observed at 200,000 ng/mL of Bevacizumab | ||||||||||||||||||||||||||||||
| Kit Contents |
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| Storage | The unopened kit is stable for at least 12 months from the date of manufacture at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C. |
Assay Principle
| Assay Principle | Bevacizumab Pharmacokinetic ELISA Kit utilizes a sandwich ELISA assay format with a pair of anti-idiotypic monoclonal antibodies for capture and detection. When standards or samples are added to the capture plate, the Bevacizumab in the sample can be captured on the plate coated with the Bevacizumab capture antibody. The Biotin Anti-Bevacizumab Antibody is then added to interact with the Bevacizumab bound on the plate. Streptavidin-Horseradish Peroxidase conjugate (Streptavidin-HRP) is added to interact with the Biotin Anti-Bevacizumab Antibody. After the washing steps, the addition of 3,3,5,5-Tetramethylbenzidine solution (TMB Solution) leads to the development of a blue color. The reaction is stopped by adding Stop Solution, causing the color to change from blue to yellow. The intensity of the resulting color can be measured at 450 nm and 630 nm using a microplate reader. The quantity of Bevacizumab in the sample is accurately determined by comparing it against a Bevacizumab standard curve. |
| Reference | 1. International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use. ICH HarmonisedGuideline, Bioanalytical Method Validation M10 (2019). 2. US FDA. Bioanalytical Method Validation Guidance for Industry (2018). 3. European Medicines Agency. Guideline on Bioanalytical Method Validation(2011). 4. |
For research use only. Not intended for human and animal therapeutic or diagnostic use.
| SKU | GSCRPT-L00969 |
|---|---|
| Featured | No |
| Supplier Part Number | L00969 |
| UM | EA |
| UNSPSC | 41116126 |
| Manufacturer | GenScript USA Inc |
| Temperature | The unopened kit is stable for at least 12 months from the date of manufacture at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C. |
| ProductLine | GSCRPT |
| Qty | 96 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 7 |
| Hazardous | Y |
| Energy Star | No |
| Green | No |
| ACT Ecolabel | No |
| Controlled | N |