Revvity Lance Eu-W1024-Itc Chela

Revvity Lance® Eu-W1024-Itc Chelate Is Optimized For The Labeling Of Proteins And Peptides At Least One Primary Aliphatic Amino Group. The Labeled Compound Can Be Used In Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer (Tr-Fret) Assays (Lance® Assays). Isothiocyanate (Itc) Activated Chelates React Primarily With Free Amine Groups Such As Ones A N-Terminus Of Protein Or Lysine Residues. Lance® W1024 Series Chelates Are Preferred In Most Hts Applications Due To Their Lower Fluorescent Background In Tr-Fret Assays. For Most Lance Applications The W1024 Series Of Eu Chelates With Active Group Can Be Used. Usable Ph Range 5-11. Under Neutral Conditions W1024 Remains Stable In Edta Concentrations Up To 20 Mmol/L. Allophycocyanin (Apc)- Or Ulight-Labeled Compounds Can Be Used As Acceptors For Lance® Tr-Fret Assays. Lance® (Lanthanide Chelate Excite) And Lance® Ultra Are Our Tr-Fret (Time-Resolved Fluorescence Resonance Energy Transfer), Homogeneous (No Wash) Technologies. One Molecule Of Interest Is Labeled With A Donor Fluorophore (A Lance Europium Chelate) And The Second Molecule Is Labeled With An Acceptor Fluorophore [Ulight Dye, Allophycocyanin (Apc)]. Upon Excitation At 320 Or 340 Nm, Energy Can Be Transferred From The Donor Europium Chelate To The Acceptor Fluorophore If Sufficiently Close For Fret (~10 Nm). This Results In The Emission Of Light At 665 Nm (For Ulight Dye And Apc) - PEGSK (Additional S&H Or Hazmat Fees May Apply)