Revvity Amyloid 1-42 (H.Spcf) (H

Revvity One Antibody Is Specific To The -Secretase Cleavage Site At The N-Terminus: Mouse Monoclonal Antibody, Clone Number 82e1. The Second Antibody Is Specific To The C-Terminus: Mouse Monoclonal Antibody, Clone Number 12f4. Formats: Our 500 Assay Point Kit Allows You To Run 500 Wells In 96-Well Or 384-Well Format, Using A 50 L Reaction Volume (5 L Of Sample). Our 5,000 Assay Point Kit Allows You To Run 5,000 Wells In 96-Well Or 384-Well Format, Using A 50 L Reaction Volume (5 L Of Sample). Features: No-Wash Steps, No Separation Steps Elisa Alternative Technology Sensitive Detection Broad Sample Compatibility Small Sample Volume Results In Less Than 3 Hours Half The Time Of An Elisa Assay Amyloid Beta (A) Is A Short Peptide Derived From The Proteolysis Of A Larger Transmembrane Molecule, The Amyloid Precursor Protein (App). The - And -Secretases Cleave The Respective N- And C-Terminal Ends Of The A Sequence, Liberating The A Peptide From App. A40 Is The Major Species Of A Produced By Neurons And Other Cells, And Accounts For Over 70% Of Total A Produced, While The Remaining 10-20% Is Comprised Of The Longer A42, And Other Species. A42 Has A Greater Propensity To Form Aggregates Or Fibrils And Also Has A Greater Neuronal Toxicity In Tissue Culture Models Than A40, Implying That A42 Is A More Important Factor In Alzheimers Disease (Ad) Pathogenesis And Plaque Formation. Levels Of A42 In Cerebrospinal Fluid Are Decreased In The Majority Of Ad Subjects (Probably Due To Its Aggregation Into Plaques), Making It An Important Biomarker For This Disease. Alphalisa Technology Allows The Detection Of Molecules Of Interest In A No-Wash, Highly Sensitive, Quantitative Assay. In An Alphalisa Assay, A Biotinylated Anti-Analyte Antibody Binds To The Streptavidin-Coated Donor Beads While Another Anti-Analyte Antibody Is Conjugated To Alphalisa Acceptor Beads. In The Presence Of The Analyte, The Beads Come Into Close Proximity. The Excitation Of The Donor Beads Causes The Release Of Singlet Oxygen Molecules That Triggers A Cascade Of Energy Transfer In The Acceptor Beads, Resulting In A Sharp Peak Of Light Emission At 615 Nm - PEGSK (Additional S&H Or Hazmat Fees May Apply)