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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ca, H, R, Rb | IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-382 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Na+/K+ ATPase β-1 Antibody, clone C464.8 |
| Overview | Clone C464.8 is also referred to as clone 8A |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein g Purified immunoglobulin in Protein g Purified immunoglobulin in PBS with 0.05% sodium azide before the addition of glycerol to 30% |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Na+/K+ ATPase β-1 Antibody, clone C464.8 is validated for use in IH, WB for the detection of Na+/K+ ATPase β-1. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla. |
| Clone | C464.8 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes the β-1 subunit isoform of Na+/K+. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 3 subunit. |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P13637 # This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. SIZE: 1013 amino acids; 111749 Da SUBUNIT: Composed of three subunits: alpha (catalytic), beta and gamma. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. DISEASE: SwissProt: P13637 # Defects in ATP1A3 are the cause of dystonia-12 (DYT12) [MIM:128235]; also known as rapid-onset dystonia parkinsonism (RDP). DYT12 is an autosomal dominant form of dystonia- parkinsonism characterized by an unusually rapid evolution of signs and symptoms. Affected persons develop dystonia and parkinsonism between 14 and 45 years of age. The sudden onset of symptoms over hours to a few weeks, often associated with physical or emotional stress, suggests a trigger initiating a nervous system insult resulting in permanent neurologic disability. SIMILARITY: SwissProt: P13637 ## Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily. |
| Molecular Weight | broad band at an apparent molecular weight of 48-55 kDa due to glycosylation |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated in immunoblot on rat brain, rat heart or rat kidney microsomal preparations |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |