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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Rb | IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-443 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-VEGF Antibody, clone JH |
| Alternate Names |
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| Background Information | The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. Flt3 is also expressed the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1k in buffer containing 10 mM PBS, pH 7.4. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-VEGF Antibody, clone JH is validated for use in IH(P) for the detection of VEGF. |
| Key Applications |
|
| Applications Not Recommended |
|
| Application Notes | Immunohistochemistry: This lot of antibody detected VEGF in formalin fixed, paraffin embedded, human.tissues, using a 1:50 dilution incubated overnight at 4°C. Immunoprecipitation: 2 µg of this antibody has been reported to immunoprecipitate VEGF from 1 mg of protein lysate. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant human VEGF121. Clone JH121 |
| Clone | clone JH |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes 34-54 kDa isoforms of Vascular Endothelial Growth Factor/Vascular Permeability Factor (VEGF/VPF). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, rabbit, mouse, and rat. Other species not tested. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. |
| Gene Symbol |
|
| Purification Method | Protein G Chromatography |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P15692 # Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. SIZE: 232 amino acids; 27042 Da SUBUNIT: Homodimer; disulfide-linked. Also found as heterodimer with PlGF (By similarity). SUBCELLULAR LOCATION: Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. TISSUE SPECIFICITY: The VEGF189, VEGF-165 and VEGF-121 isoforms are widely expressed, whereas the VEGF206 and VEGF-145 are uncommon. SIMILARITY: SwissProt: P15692 ## Belongs to the PDGF/VEGF growth factor family. |
| Molecular Weight | 34-54 kDa isoforms |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Biological Blockade: Incubation of HUVEC cells with 5 µg/mL of a previous lot and 5 ng/mL of VEGF (Catalog # 01-185) has shown growth inhibition of 20-70%. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |