Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, R | WB, IP, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-474 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-GABA A Receptor β2/3 Antibody |
| Alternate Names |
|
| Background Information | The neurotransmitter GABA (gamma-aminobutyric acid) binds to specific transmembrane receptors in the plasma membrane of both pre- and postsynaptic neurons. Three general classes of GABA receptors are known: GABAA and GABAC (ionotropic) receptors and GABAB (metabotropic) receptors. The GABAA (gamma-Aminobutyric Acid type A) receptor is a multiprotein complex that acts as a ligand-gated chloride channel. These receptors are hetero-oligomers, for which 6 subunits are currently known (a, b, g, d, e and r). There are at least 3 different isoforms of the beta subunit, and receptor subunit expression varies during development. Additionally, GABAA receptors are important in regulating the reinforcing properties of alcohol, and expression of the alpha1 and beta 2/3 subunits increase upon chronic exposure to alcohol, and may be potential therapeutic targets for alcohol dependency. The beta 2 subunit is required for receptor assembly and surface expression, while mice with an inactivated beta3 subunit exhibit epilepsy and cleft palate. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-GABA A Receptor β2/3 Antibody, is an antibody against GABA A Receptor β2/3 for use in WB, IP, IH. |
| Key Applications |
|
| Application Notes | Immunoprecipitation: 4 μg of a previous lot immunoprecipitated the GABAA Receptor β2 and β3 subunits from 500 μg of rat brain microsomal tissue extract. Due to interference of the IgG heavy chain, the immunoprecipitated GABAA receptor complex was confirmed using a polyclonal anti-GABAA receptor α1 subunit (Catalog # 06-868). Immunohistochemistry: This antibody has been reported to detect the GABAA Receptor β2 and β3 subunits in paraffin embedded and frozen rat brain sections which had been fixed with 4% paraformaldehyde or 0.1% glutaraldehyde and permeabilized with Triton X-100. |
| Biological Information | |
|---|---|
| Immunogen | Affinity-purified GABAA receptor isolated from bovine brain. Clone 62-3G1 |
| Clone | clone 62-3G1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Specific for the GABAA Receptor β2 subunit, MW ~55 kDa and β3 subunit, MW ~57 kDa. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P14867 # GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. SIZE: 456 amino acids; 51802 Da SUBUNIT: Binds UBQLN1 (By similarity). Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. DISEASE: SwissProt: P14867 # Defects in GABRA1 are a cause of juvenile myoclonic epilepsy (JME) [MIM:606904]. JME is a common epileptic syndrome characterized by afebrile seizures, onset in adolescence (rather than in childhood) and myoclonic jerks. & Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. ECA4 is a subtype of idiopathic generalized epilepsy (IGE) characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop. Absence seizures may either remit or persist into adulthood. SIMILARITY: SwissProt: P14867 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family. |
| Molecular Weight | Varies |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by western blot on rat brain microsomal preparation. Western Blot Analysis: 0.5-2 μg/mL of this antibody detected the GABAA Receptor β2 and β3 subunits from 20 μg of rat brain microsomal preparation (Catalog # 12-144). |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2°C to 8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |