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The lactose operon repressor binds to a regulatory element, an operator gene, to form a genetic switch, the lac operon. This switch negatively regulates the expression of structural genes that follow the site of the operator gene. Inducers may bind to the repressor to reduce or eliminate the affinity of the repressor for its operator, and thereby allow for transcription of respective structural genes. Conversely, other effector molecules may increase the binding affinity of the repressor for its operator and thereby inhibit gene transcription. The inducer or effector molecules that associate with a repressor serve as a chemical signal, which monitors the metabolic state of protein(s) expressed by their respective genes.
Product Information
Format
Purified
Control
Rosetta BL21 (DE3) competent E. Coli cell lysate.
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide (This antibody is purified from supernatent.).
Quality Level
MQ100
Applications
Application
Anti-LacI Antibody, clone 9A5 is an antibody against LacI for use in Western Blotting.
Key Applications
Western Blotting
Biological Information
Immunogen
TrpE fused with full-length E. Coli LacI.
Clone
9A5
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
E. coli
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_414879
Gene Symbol
lacI
Purification Method
Protein g Purified
UniProt Number
P03023
Molecular Weight
~38 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in Rosetta™ BL21 (DE3) E. Coli cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected LacI in 10 µg of Rosetta BL21 (DE3) E. Coli cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
