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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Yeast (S. cerevisiae) | IP, WB | M | Culture Supernatant | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-545 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-PP2A Antibody, C subunit, clone 7A6 |
| Background Information | Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform (UniProt: P62714; also known as EC:3.1.3.16, PP2A-β) is encoded by the PPP2CB gene (Gene ID: 5516) in human. Phosphorylation and dephosphorylation of structural and regulatory proteins are major intracellular control mechanisms in eukaryotes. Protein phosphatase 2A is a major phosphoserine and phosphothreonine protein phosphatase present in all eukaryotic cells. It is involved in the regulation of several processes, including signaling cascades, cell cycle regulation, apoptosis, and development. It consists of a 36 kDa catalytic subunit (PP2A C), that is complexed with a 65 kDa regulatory subunit (PR65). This dimer associates with variable regulatory subunits of 55 kDa (PR55), 72 kDa (PR72), or 130 kDa (PR130). These various regulatory subunits affect substrate specificity and the subcellular distribution of PP2A. The PR72 unit contains a potential nuclear localization signal in its primary sequence, which may help PP2A to translocate to the nucleus. PP2A C has a highly conserved C terminus that plays an important role in regulating its phosphatase activity. In mammalian cells about 90% of PP2A C is present in the carboxymethylated state. This carboxyl methylation is believed to stimulate PP2A activity and promote PP2A heterotrimeric complex assembly. Dysregulation of PP2A methylation is reported to have important biological consequences. Reduced PP2A methylation has been linked to an increased risk of Alzheimer’s disease. Clone 7A6 is generated against a sequence from the C-terminal region of PPA2 C and it preferentially recognizes the non-methylated form of the enzyme and is not suitable for evaluating PP2A activity and holoenzyme composition. (Ref.: Frohner, IE., et Al. (2020). Science Sig. 13(616); eaax6490). |
| Product Information | |
|---|---|
| Format | Culture Supernatant |
| Control |
|
| Presentation | Cell culture supernatant containing 30% glycerol and 0.05% sodium azide as a preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PP2A Antibody, C subunit, clone 7A6 preferentially recognizes the non-methylated form of the enzyme and is not suitable for evaluating PP2A activity and holoenzyme composition. (Ref.: Frohner, IE., et Al. (2020). Science Sig. 13(616); eaaz6490). |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | peptide (C-bARRTPDYFL) corresponding to amino acids 302-309 of human PP2A catalytic subunit |
| Clone | 7A6 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes the catalytic subunit of PP2A. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P67775 # PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity). SIZE: 309 amino acids; 35594 Da SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B/PR72/PR130/PR59 and R5/B/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. SUBCELLULAR LOCATION: Cytoplasm. PTM: Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. & Phosphorylation of either threonine (by autophosphorylation- activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. SIMILARITY: SwissProt: P67775 ## Belongs to the PPP phosphatase family. PP-1 subfamily. |
| Molecular Weight | 36 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on RIPA lysates from A431 cells |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |