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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, PO, R, Rb, Xn | IHC, IP, WB | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-592 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-PP2A Antibody, B subunit, clone 2G9 |
| Background Information | Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform (UniProt: P62714; also known as EC:3.1.3.16, PP2A-β) is encoded by the PPP2CB gene (Gene ID: 5516) in human. Phosphorylation and dephosphorylation of structural and regulatory proteins are major intracellular control mechanisms in eukaryotes. Protein phosphatase 2A is a major phosphoserine and phosphothreonine protein phosphatase present in all eukaryotic cells. It is involved in the regulation of several processes, including signaling cascades, cell cycle regulation, apoptosis, and development. It consists of a 36 kDa catalytic subunit (PP2A C), that is complexed with a 65 kDa regulatory subunit (PR65). This dimer associates with variable regulatory subunits of 55 kDa (PR55), 72 kDa (PR72), or 130 kDa (PR130). These various regulatory subunits affect substrate specificity and the subcellular distribution of PP2A. The PR72 unit contains a potential nuclear localization signal in its primary sequence, which may help PP2A to translocate to the nucleus. PP2A C has a highly conserved C terminus that plays an important role in regulating its phosphatase activity. In mammalian cells about 90% of PP2A C is present in the carboxymethylated state. This carboxyl methylation is believed to stimulate PP2A activity and promote PP2A heterotrimeric complex assembly. Dysregulation of PP2A methylation is reported to have important biological consequences. Reduced PP2A methylation has been linked to an increased risk of Alzheimer’s disease. Clone 2G9 is raised against a sequence from the C-terminal region of B55α subunit. It recognizes α and δ B subunits and can coimmunoprecipitate trimeric PP2A holoenzyme consisting of PP2A B, PP2A A, and exclusively the methylated form of PP2A C subunit. It is shown to immunoprecipitate high amounts of B-containing trimers from wild-type cells. (Ref.: Frohner, IE., et Al. (2020). Science Sig. 13(616); eaax6490). |
| Product Information | |
|---|---|
| Format | Ascites |
| Presentation | 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide and glycerol to 30% |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This antibody recognizes α and δ B subunits of PP2A and can coimmunoprecipitate trimeric PP2A holoenzyme consisting of PP2A B, PP2A A, and exclusively the methylated form of PP2A C subunit. It is shown to immunoprecipitate high amounts of B-containing trimers from wild-type cells. (Ref.: Frohner, IE., et Al.(2020). Science Sig. 13(616); eaax6490). |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Peptide corresponding to residues 398-411 of human PP2A, B subunit |
| Clone | clone 2G9 |
| Host | Mouse |
| Specificity | PP2A B subunit, isoform reactivity is α > δ,β > γ |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Ascites |
| UniProt Number |
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| UniProt Summary | Function: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Subunit structure: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B/PR72/PR130/PR59 and R5/B/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Tissue specificity: In all tissues examined. Post-translational modification: The N-terminus is blocked. Sequence similarities: Belongs to the phosphatase 2A regulatory subunit B family. Contains 7 WD repeats. |
| Molecular Weight | 55kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on RIPA lysates from non-stimulated A431 cells or mouse 3T3/A31 cells |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |