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Millipore Detect Brdu Also Known As Bromodeoxyuridine With Anti-Brdu Antibody, Clone Bu-1 (Mouse Monoclonal Antibody), That Has Been Demonstrated To Work In Icc. - Mill (Additional S&H or Hazmat Fees May Apply)
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Product Information
Format
Culture Supernatant
Presentation
4 vials, each vial containing mouse culture supernatant lyophilized from 2.5 mL. The supernatant contains mycoplasma, which contribute enzymes that facilitate antibody binding. Lyophilized powder.
Quality Level
MQ100
Applications
Application
Detect BrdU also known as Bromodeoxyuridine with Anti-BrdU Antibody, clone BU-1 (Mouse Monoclonal Antibody), that has been demonstrated to work in ICC.
Key Applications
Immunocytochemistry
Biological Information
Immunogen
5-iodouridine:ovalbumin conjugate
Clone
clone BU-1
Host
Mouse
Specificity
5-Bromo-2-deoxyuridine
Isotype
IgG2a
Species Reactivity
All
Antibody Type
Monoclonal Antibody
Purification Method
Concentrated culture supernatant
Molecular Weight
none given
Product Usage Statements
Quality Assurance
A representative lot was evaluated by immunocytochemistry on HeLa cells. A 1:2-1:4 dilution from a representative lot showed positive immunostaining for Anti-BrdU in HeLa cells fixed with 95% ethanol, 5% glacial acetic acid. This lot was tested using the DNA Replication Assay Kit (Catalog # 17-316).
Technical Tip: This antibody detects 5-Bromo-2- deoxyuridine incorporated into double-stranded DNA. DNA denaturation is not required.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
