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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-647 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Tyrosinase Antibody, clone T311 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30% |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Tyrosinase Antibody, clone T311 is validated for use in WB, IH for the detection of Tyrosinase. |
| Key Applications |
|
| Application Notes | Not recommended for Immunoprecipitation |
| Biological Information | |
|---|---|
| Immunogen | Purified recombinant Tyrosinase corresponding to residues 5-456 of human Tyrosinase |
| Clone | T311 |
| Host | Mouse |
| Specificity | Tyrosinase |
| Isotype | IgG2a |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein g Chromatography |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P14679 # This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone. COFACTOR: Binds 2 copper ions per subunit. SIZE: 529 amino acids; 60393 Da SUBCELLULAR LOCATION: Melanosome membrane; Single-pass type I membrane protein. DISEASE: SwissProt: P14679 # Defects in TYR are the cause of oculocutaneous albinism type IA (OCA-IA) [MIM:203100]. OCA-I, also known as tyrosinase negative oculocutaneous albinism, is an autosomal recessive disorder characterized by absence of pigment in hair, skin and eyes. OCA-I is divided into 2 types: type IA, characterized by complete lack of tyrosinase activity due to production of an inactive enzyme, and type IB characterized by reduced activity of tyrosinase. OCA-IA patients presents with the life-long absence of melanin pigment after birth and manifest increased sensitivity to ultraviolet radiation and to predisposition to skin cancer. & Defects in TYR are the cause of oculocutaneous albinism type IB (OCA-IB) [MIM:606952]; also known as albinism yellow mutant type. OCA-IB patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal- to-moderate amounts of cutaneous and ocular pigment. & Defects in TYR are the cause of oculocutaneous albinism type I temperature-sensitive (OCA-ITS) [MIM:606952]. OCA-ITS patients have white axillary and scalp hair and pigmented arm and leg hair. SIMILARITY: SwissProt: P14679 ## Belongs to the tyrosinase family. |
| Molecular Weight | 70-80kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on RIPA lysates from SK-MEL-19 cells |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |