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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-652 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-MUC1/episialin Antibody, clone 214D4 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30% |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MUC1/episialin Antibody, clone 214D4 detects level of MUC1/episialin & has been published & validated for use in FC, IH. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Fusion protein, human b-gal-episialin/MUC1 repeat domain |
| Clone | clone 214D4 |
| Host | Mouse |
| Specificity | MUC1/episialin |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined. |
| Gene Symbol |
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| Purification Method | Protein g Chromatography |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P15941 # Isoform 7 behaves as a receptor and binds the secreted isoform 5. The binding induces the phosphorylation of the isoform 7, alters cellular morphology and initiates cell signaling. Can bind to GRB2 adapter protein. SIZE: 1255 amino acids; 122072 Da SUBUNIT: The cleaved form (only the isoform 1) of MUC1 forms a tight heterodimer with the released C-terminal peptide (which is first secreted to be extracellular). SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 5: Secreted. & Isoform 9: Secreted. TISSUE SPECIFICITY: Expressed on ductal epithelial cells and on activated T-cells. Aberrantly glycosylated forms expressed in human epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 expressed only in tumoral cells. PTM: Highly glycosylated (N- and O-linked carbohydrates and sialic acid). In the 20 amino acid tandem repeat positions 5 (Ser), 6 (Thr), 14 (Thr), 15 (Ser) and 19 (Thr) are O-glycosylated (GalNAc). The average density of O-glycosylated sites within repeat peptides varies with cell differentiation from about 50% in lactation-associated MUC1 to over 90% in a variety of breast cancer cells. & Isoform 1 and isoform 7 undergoes transphosphorylation on serine and tyrosine residues. SIMILARITY: SwissProt: P15941 ## Contains 1 SEA domain. |
| Molecular Weight | none given |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunohistochemistry |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |