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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Vrt | ICC, IHC, IP, WB, ChIP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-689 |
| Replaces | MABE479 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-HP1α Antibody, clone15.19s2 |
| Alternate Names |
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| Background Information | Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and each contains a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated on Lys9, a histone "mark" closely associated with centromeric heterochromatin. The variable hinge region binds both RNA and DNA in a sequence-independent manner. The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases and the p150 subunit of chromatin-assembly factor-1 (CAF1). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells. HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination and sumoylation, suggesting multiple means of regulation. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% Sodium Azide before the addition of glycerol to 30%. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-HP1α Antibody, clone15.19s2 is a Mouse Monoclonal Antibody for detection of HP1α also known as HP1Hs alpha, Heterochromatin protein 1 homolog alpha & has been validated in ChIP, ICC, IHC, IP & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: A previous lot of this antibody has been reported by an independent laboratory to detect HP1a in NIH/3T3 nuclei (Ayyanathan, K., 2003). Immunoprecipitation/Chromatin Immunoprecipitation: A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate HP1α from nuclear extracts and formalin-cross-linked chromatin (Ayyanathan, K., 2003). |
| Biological Information | |
|---|---|
| Immunogen | GST fusion protein corresponding to residues 2-191 of human HP1α protein. |
| Clone | 15.19s2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes HP1α |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing.[supplied by OMIM] |
| Gene Symbol |
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| Purification Method | Protein g Chromatography |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P45973 # Component of heterochromatin. Recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. Can interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. SIZE: 191 amino acids; 22225 Da SUBUNIT: Interacts with SUV420H1 and SUV420H2 (By similarity). Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at Lys-9. Interacts with MIS12 and C20orf127. SUBCELLULAR LOCATION: Nucleus. Centromere. Note=Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. PTM: Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper- phosphorylated during mitosis. SIMILARITY: SwissProt: P45973 ## Contains 2 chromo domains. |
| Molecular Weight | 29 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by western blot in acid-extracted HeLa cell lysates. Western Blot Analysis: 0.5-2 µg/mL of this lot detected HP1α protein in acid-extracted HeLa cell lysates. |
| Sales Restrictions | Due to license agreement restrictions, this product cannot be purchased for resale. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |