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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ICC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-718 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-PML Antibody, clone 36.1-104 |
| Background Information | Promyeloctic Leukemia Gene product (PML) is a nuclear protein with growth-suppressive properties originally identified in the context of the PML-retinoic receptor alpha (RAR alpha) fusion protein of acute promyelocytic leukemia. PML localizes within distinct nuclear structures, called nuclear bodies, which are disrupted by the expression of PML-RAR alpha. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein g Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide as a preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect PML also known as Promyelocytic Leukemia Protein with Anti-PML Antibody, clone 36.1-104 (Mouse Monoclonal Antibody), that has been demonstrated to work in ICC & WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | 6His fusion protein corresponding to mouse PML (promyelocytic leukemia protein) |
| Clone | 36.1-104 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes PML. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the proteins central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P29590 # Probable transcription factor. May play an important role in recruitment of ELF4 into PML nuclear bodies. SIZE: 882 amino acids; 97517 Da SUBUNIT: Interacts with SIRT1, TOPBP1, TRIM27 and TRIM69. Interacts with the C-terminus of ELF4. Interacts with Lassa virus Z protein and rabies virus phosphoprotein (By similarity). SUBCELLULAR LOCATION: Nucleus. Note=Localized to PML nuclear bodies. PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. DISEASE: SwissProt: P29590 # A chromosomal aberration involving PML may be a cause of acute promyelocytic leukemia (APL). Translocation t(15;17)(q21;q21) with RARA. The PML breakpoints (type A and type B) lie on either side of an alternatively spliced exon. SIMILARITY: SwissProt: P29590 ## Contains 2 B box-type zinc fingers. & Contains 1 RING-type zinc finger. |
| Molecular Weight | 90-106 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by immunoblot on RIPA lysates from mouse embryo fibroblast (MEF1) cells |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |