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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | ICC, IF, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-807 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-phospho-CREB (Ser133) Antibody, clone 634-2 |
| Alternate Names |
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| Background Information | CREB is a β ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse IgG1κ in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Liquid at -20ºC. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-phospho-CREB (Ser133) Antibody, clone 634-2 (mouse monoclonal antibody) validated in ICC, IF, WB to detect phospho-CREB (Ser133) also known as active transcription factor CREB, cAMP responsive element binding protein 1. |
| Key Applications |
|
| Application Notes | Beadlyte Specificity Assay: 1:500 to 1:32,000 dilutions of this lot detected CREB peptide containing phosphoserine 133. The antibody did NOT recognize the nonphosphorylated peptide of the same sequence Immunofluorescence / Immunocytochemistry: This antibody has been reported by an independent laboratory to detect phospho-CREB (Ser133) using immunofluorescence. |
| Biological Information | |
|---|---|
| Immunogen | A proprietary immunogen based on the amino acid sequence containing phospho-serine corresponding to residue 133 of human CREB. |
| Epitope | Ser133 |
| Clone | 634-2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Phospho-CREB (Ser133). |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Mouse and rat. Broad species cross-reactivity expected, based on sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. |
| Gene Symbol |
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| Modifications |
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| Purification Method | Protein g Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P16220 # This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. SIZE: 341 amino acids; 36688 Da SUBUNIT: Binds DNA as a dimer. Interacts PPRC1 and with HTLV-1 Tax-1. SUBCELLULAR LOCATION: Nucleus. PTM: Stimulated by phosphorylation. Phosphorylation of both Ser- 133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP and EP300 binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. SIMILARITY: SwissProt: P16220 ## Belongs to the bZIP family. & Contains 1 bZIP domain. & Contains 1 KID (kinase-inducible) domain. |
| Molecular Weight | 43 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by western blot on RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells. Western Blot Analysis: A 1:1,000 to 1:4,000 dilution of this lot detected phospho-CREB (Ser 133) in RIPA lysates from forskolin treated rat PC-12 and mouse 3T3/A31 cells (Figure A). |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |