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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M, Po, B, Av, Gp, H | ICC, IF, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-829 |
| Replaces | 04-1117 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-α-Tubulin Antibody, clone DM1A |
| Alternate Names |
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| Background Information | Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide and 30% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-α-Tubulin Antibody, clone DM1A is an antibody against α-Tubulin for use in IC, IF & WB. |
| Key Applications |
|
| Application Notes | Immunofluorescence: (Breitling, F., 1986) |
| Biological Information | |
|---|---|
| Immunogen | Purified human brain α-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSVEGEGEEEGEE). |
| Epitope | a.a. 426-450 |
| Clone | DM1A |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes α-Tubulin, clone DM1A, MW 50-60 kDa. The epitope recognized by this antibody has been mapped to amino acids 426-450. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q71U36 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. SIZE: 451 amino acids; 50136 Da SUBUNIT: Dimer of alpha and beta chains. PTM: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively (By similarity). SIMILARITY: SwissProt: Q71U36 ## Belongs to the tubulin family. |
| Molecular Weight | 50-60 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by western blot on non-stimulated A431 lysate. Western Blot Analysis: 0.1-1 µg/mL of this lot detected α- Tubulin in RIPA lysates from A431, L6 cells and rat whole brain tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |