Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IP, ICC, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-844 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-PLK1 Antibody, clone 35-206 |
| Alternate Names |
|
| Background Information | Polo-like Kinase 1 (PLK-1) is a serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Expressed PLK-1 protein accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Protein A purified mouse IgG2b in buffer containing 0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol. Store at -20°C. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PLK1 Antibody, clone 35-206 detects level of PLK1 & has been published & validated for use in WB, IP, IC, IH. |
| Key Applications |
|
| Application Notes | Immunoprecipitation: An independent laboratory has reported that a previous lot of this antibody can be used to immunoprecipitate PLK1. Immunohisto/immunocytochemistry: An independent laboratory reported that 1 μg/mL of a previous lot of this antibody showed positive immunostaining for PLK1 in paraformaldehyde treated, Triton X-100 fixed cells. |
| Biological Information | |
|---|---|
| Immunogen | 6His-tagged full length fusion protein corresponding to human polo-like kinase 1 (PLK1). Also called serine-threonine protein kinase PLK. |
| Clone | clone 35-206 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | PLK1 |
| Isotype | IgG2b |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P53350 # May be required for cell division and may have a role during G1 or S phase. SIZE: 603 amino acids; 68255 Da SUBUNIT: Interacts with CEP170 and EVI5. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Placenta and colon. DEVELOPMENTAL STAGE: Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. PTM: Catalytic activity is enhanced by phosphorylation of Thr-210 and/or Ser-137. & Autophosphorylation and phosphorylation of Ser-137 are not significant events during activation of PLK1 in M phase. SIMILARITY: SwissProt: P53350 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. & Contains 2 POLO box domains. & Contains 1 protein kinase domain. |
| Molecular Weight | 64 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by western blot on RIPA lysates from 3T3/A31 cells. Western Blot Analysis: 0.5-2 μg/mL of this antibody detected PLK1 in RIPA lysates from 3T3/A31 cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |