| Replacement Information | |
|---|---|
| Replacement Information | 06-574 is a recommended replacement for CBL770 |
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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IF, IHC, IP, WB, ChIP | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-939 |
| Replaces | CBL770 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-LSD1/BHC110 Antibody |
| Alternate Names |
|
| Background Information | Histone demethylase that specifically demethylates Lys-4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri-methylted Lys-4 of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 Lys-4 on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate Lys-9 of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. |
| Product Information | |
|---|---|
| Format | Ascites |
| Control |
|
| Presentation | Mouse monoclonal in buffer containing 0.05% sodium azide and 30% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-LSD1/BHC110 Antibody is a Mouse Monoclonal Antibody for detection of LSD1/BHC110 also known as BRAF35-HDAC complex protein BHC110, lysine (K)-specific demethylase 1 & has been validated in ChIP, IF, IHC, IP & WB. |
| Key Applications |
|
| Application Notes | Chromatin Immunoprecipitation: This antibody has been reported by an independent laboratory to immunoprecipitate LSD1 from chromatin. Immunoprecipitation: Recommended. Immunohistochemistry: This antibody has been reported by an independent laboratory to detect LSD1 using paraffin-embedded tissues. (Metzger, E., 2005) Immunofluorescence: Recommended. |
| Biological Information | |
|---|---|
| Immunogen | GST fusion protein corresponding to residues 23-138 of human LSD1. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Recognizes LSD1, Mr 110 kDa. |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternate splicing has been observed; however, not all transcript variants have been fully characterized. |
| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: O60341 # Histone demethylase that specifically demethylates Lys- 4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri- methylated Lys-4 of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 Lys-4 on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate Lys- 9 of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. COFACTOR: FAD. SIZE: 852 amino acids; 92903 Da SUBUNIT: Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR). SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Ubiquitously expressed. DOMAIN: SwissProt: O60341 The SWIRN domain may act as an anchor site for a histone tail. SIMILARITY: Belongs to the flavin monoamine oxidase family. & Contains 1 SWIRM domain. |
| Molecular Weight | 110 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by western blot. Western Blot Analysis: 1:500-1:2000 dilution of this lot detected LSD1 in RIPA lysates from HeLa cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |