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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-940 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-INCENP Antibody, clone 58-217 |
| Product Information | |
|---|---|
| Format | Purified |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect INCENP with Anti-INCENP Antibody, clone 58-217 (Mouse Monoclonal Antibody), that has been shown to work in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | 6His tagged fusion protein corresponding to residues 231-398 of human Inner Centromere Protein (INCENP). Clone 58-217. |
| Clone | clone 58-217 |
| Host | Mouse |
| Specificity | INCENP |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | NOT mouse or rat. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and passenger, or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term passenger proteins encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et Al., 1999 [PubMed 10369859]).[supplied by OMIM] |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q9NQS7 # Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. SIZE: 923 amino acids; 106114 Da SUBUNIT: Homodimer or heterodimer. Interacts with H2AFZ (By similarity). Interacts with CBX3. Interacts with tubulin beta chain. Interacts with AURKB and AURKC. Component of the CPC at least composed of BIRC5/survivin, CDCA8/borealin, INCENP and AURKB/Aurora-B. Interacts with EVI5. SUBCELLULAR LOCATION: Centromere. Spindle. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes. |
| Molecular Weight | 135-155kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at -20°C from date of shipment |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |