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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Vrt, Yeast (S. cerevisiae) | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 05-944 |
| Replaces | 04-454 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Ubiquitin Antibody, clone P4D1-A11 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | 100 μg of protein g purified mouse IgG in 100 μL of 70% storage buffer (0.014M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide) and 30% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Ubiquitin Antibody, clone P4D1-A11 is an antibody against Ubiquitin for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | total bovine ubiquitin |
| Clone | P4D1-A11 |
| Host | Mouse |
| Specificity | Ubiquitin, Mr = 8 kDa, and several ubiquitinated proteins |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimers and Down syndrome. |
| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P62988 # Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous fonctions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. SIZE: 76 amino acids; 8565 Da SUBCELLULAR LOCATION: Cytoplasm. Nucleus. PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly. SIMILARITY: SwissProt: P62988 ## Belongs to the ubiquitin family. MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a). |
| Molecular Weight | 8 kDa and higher |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot of human recombinant ubiquitin (Catalog # 12-558), and ubiquitinated proteins in acid extracts from HeLa cells |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at -20°C from date of shipment |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |