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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, Mplex, PIA, DB, ChIP-seq, ChIP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-329 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Anti-acetyl-Histone H4 (Lys16) Antibody |
| Alternate Names |
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| Background Information | Histone H4, Acetylated on Lysine 16 (UniProt: P62805; also known as H4K16Ac, Histone H4 (acetyl K16), H4 histone family, member A, H4a) is encoded by the HIST1H4A (also known as H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, HIST1H4I, H4/N, HIST1H4B) gene (Gene ID: 121504, 554313, 8294, 8359, 8360, 8361, 8362, 8363, 8364, 8365, 8366, 8367, 8368, 8370) in human. Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H4 features a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the beads on a string structure. Histone H4 are known to be acetylated on Lysine 5, 8, 12, and 16. Acetylations are important for the regulation of histone deposition, transcriptional activation, DNA replication and repair. Hyper-acetylation of the histone tails is shown to neutralize positive charge and weakens histone-DNA and nucleosome-nucleosome interactions that destabilizes chromatin structure and increases the accessibility of DNA to various DNA-binding proteins. Chromosomal aberrations involving histone H4 is a cause of B-cell non-Hodgkin lymphomas. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-acetyl-Histone H4 (Lys16) Antibody is a rabbit polyclonal antibody for detection of acetyl-Histone H4 (Lys16) also known as H4K16Ac, Histone H4 (acetyl K16 & has been published and validated in ChIP, WB, Mplex, PIA, DB, ChIP-seq. |
| Key Applications |
|
| Application Notes | Chromatin Immunoprecipitation Analysis: 1 ug from a representative lot immunoprecipitated Acetyl-Histone H4 (Lys16) in HeLa chromatin. Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Acetyl-Histone H4 (Lys16) in HeLa, A431, HUVEC, and NIH/3T3 cell lines. Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected Acetyl-Histone H4 (Lys16) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H4 acetylated on Lysine 16. |
| Epitope | N-terminus (Lys 16) |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects human Histone H4 acetylated on Lysine 16. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Broad species cross-reactivity is expected. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | 10 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in lysate from Sodium Butyrate treated HeLa cells. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Acetyl-Histone H4 (Lys16) in lysates from Sodium Butyrate treated HeLa cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8° C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |