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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP, WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-419 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-SHP-1/SHPTP-1 Antibody |
| Alternate Names |
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| Background Information | Tyrosine-protein phosphatase non-receptor type 6 (UniProt: P29350; also known as EC:3.1.3.48, Hematopoietic cell protein-tyrosine phosphatase, Protein-tyrosine phosphatase 1C, PTP-1C, Protein-tyrosine phosphatase SHP-1, SH-PTP1) is encoded by the PTPN6 (also known as HCP, PTP1C) gene (Gene ID: 5777) in human. Protein tyrosine phosphorylation plays a critical role in regulating cell growth, differentiation, and activation of terminally differentiated cells. The level of phosphorylation results from a balance between the activity of protein tyrosine kinases and protein tyrosine phosphatases. SHP-1 is a monomeric protein phosphatase that is mainly expressed in hematopoietic cells and contains two SH2 domains. Its SH2 domains interact with other cellular components to modulate its own phosphatase activity against interacting substrates. SHP-1 is localized in the cytoplasm, but it shuttles between the cytoplasm and nucleus via its association with phosphoinositide-dependent Kinase-1 (PDPK1). It modulates signaling by tyrosine phosphorylated cell surface receptors, such as KIT and EGFR. It contains two putative sites of tyrosine phosphorylation in its C-terminal region that can be phosphorylated by both receptor and non-receptor protein tyrosine kinases. SHP-1 is reported to undergo phosphorylation at tyrosine 564 by Lyn that enhances its phosphatase activity. SHP-1 is considered as a tumor suppressor and the main mechanism by which it curbs tumor development and progression is by its ability to attenuate signaling pathways that control cell proliferation, survival, migration, and invasion. (Ref.: Varone, A., et Al. (2020). Front. Oncology. 10; 935; Yoshida, K., et Al. (1999). J. Biol. Chem. 274(49); 34663-34668; Li, RY., et Al. (1995). EMBO J. 14(11); 2519-2526). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SHP-1/SHPTP-1, Cat. No. 07-419, is a rabbit polyclonal antibody that detects SHP-1 and is tested for use in Western Blotting and Immunoprecipitation. |
| Key Applications |
|
| Application Notes | Tested Applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GST-tagged recombinant Human SHP-1. Immunoprecipitation Analysis: A representative lot immunoprecipitated SHP-1 in Immunoprecipitation applications (Bruhns, P., et Al. (2000). J. Biol. Chem. 275(48); 37357-37364). Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged, full-length, recombinant human SHP-1. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects SHP-1/SHPTP1 protein. |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. |
| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P29350 # Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. SIZE: 595 amino acids; 67561 Da SUBUNIT: Monomer. Interacts with MTUS1 (By similarity). Binds PTPNS1, LILRB1 and LILRB2. Interacts with FCRL2, CD300LF and CD84. SUBCELLULAR LOCATION: Cytoplasm. Nucleus (By similarity). Note=In neurons, translocates into the nucleus after treatment with angiotensin II (By similarity). TISSUE SPECIFICITY: Isoform 1 is expressed in hematopoietic cells while isoform 2 is expressed in non-hematopoietic cells. PTM: Phosphorylated on serine and tyrosine residues. SIMILARITY: SwissProt: P29350 ## Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily. & Contains 2 SH2 domains. & Contains 1 tyrosine-protein phosphatase domain. |
| Molecular Weight | Target molecular weight ~65 kDa observed. 67.56 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A431 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SHP-1 in A431 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Recommended storage: +2°C to +8°C. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |