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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, B, M, Ca, Gp, Po | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-520 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-eNOS/NOS III Antibody, CT |
| Overview | Note: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS |
| Alternate Names |
|
| Background Information | Endothelial NOS (eNOS), also known as Nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is a nitric oxide synthase. It generates NO in blood vessels and is involved with regulating vascular function. A constitutive Ca2+ dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of Golgi bodies within cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide before the addition of glycerol to 30%. Liquid at -20°C. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-eNOS/NOS III Antibody, CT detects level of eNOS/NOS III & has been published & validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, synthetic peptide corresponding to amino acids 1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, pig, rat, and dog. |
| Epitope | C-Terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes eNOS/NOS III, Mr ~135 kDa. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human and bovine. Predicted to cross-react with mouse, guinea pig, pig, rat and dog based on sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P29474 # Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. No mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme. SIZE: 1203 amino acids; 133289 Da SUBUNIT: Homodimer. Interacts with NOSIP and NOSTRIN. SUBCELLULAR LOCATION: Cell membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. TISSUE SPECIFICITY: Platelets, placenta, liver and kidney. SIMILARITY: SwissProt: P29474 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain. ... hide » see more » |
| Molecular Weight | 135 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by western blot in RIPA lysates from HUVEC cells. Western Blot Analysis: 0.5-2 µg/mL of this lot detected eNOS/NOS III in RIPA lysates from HUVEC cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 200 µg |