The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Anti-Phospho-Pkr (Thr446) Antibody Is An Antibody Against Phospho-Pkr (Thr446) For Use In Wb. - Mill (Additional S&H or Hazmat Fees May Apply)
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Quality Level
MQ100
Applications
Application
Anti-phospho-PKR (Thr446) Antibody is an antibody against phospho-PKR (Thr446) for use in WB.
Key Applications
Western Blotting
Biological Information
Immunogen
Peptide corresponding to amino acids surrounding Thr446 of human PKR.
Host
Rabbit
Specificity
phospho-PKR
Isotype
IgG
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_002759
Gene Symbol
EIF2AK2
MGC126524
EIF2AK1
PRKR
PKR
Modifications
Phosphorylation
Purification Method
Protein A Purfied
UniProt Number
P19525
UniProt Summary
FUNCTION: SwissProt: P19525 # Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection. SIZE: 551 amino acids; 62094 Da SUBUNIT: Homodimer. Interacts with STRBP (By similarity). Interacts with DNAJC3. Inhibited by direct interaction with viral proteins such as HCV E2, HCV NS5A and influenza A NS1. Activated by the interaction with HIV-1 Tat. PTM: Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase. SIMILARITY: SwissProt: P19525 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. & Contains 2 DRBM (double-stranded RNA-binding) domains. & Contains 1 protein kinase domain.
Molecular Weight
68 kDa
Product Usage Statements
Quality Assurance
routinely evaluated by immunoblot in RIPA lysates from HeLa cells that had been stimulated with IFN alpha overnight followed by treatment with calyculin A for 15 minutes
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
