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Millipore Anti-Acetyl-Histone H3 (Lys56) Is An Antibody Targeting The Acetyl-Histone H3 (Lys56) Protein, Validated For Use In Wb & Chip. - Mill (Additional S&H or Hazmat Fees May Apply)
Histone H3 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H3 is involved with the structure of the nucleosomes of the beads on a string structure. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
Product Information
Format
Affinity Purified
Control
HeLa acid extract, sodium butyrate treated HeLa acid extract, colcemid treated HeLa acid extract, recombinant Histone H3, recombinant Histone H4, and recombinant Histone H2A
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-acetyl-Histone H3 (Lys56) is an antibody targeting the acetyl-Histone H3 (Lys56) protein, validated for use in WB & ChIP.
Key Applications
Western Blotting
DOT Blot
Chromatin Immunoprecipitation (ChIP)
Application Notes
DOT Blot (Specificity) Analysis: A representative lot detected acetyl-Histone H3 (Lys56) in an Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated acetyl-Histone H3 (Lys56) from HeLa cells.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human Histone H3 acetylated at Lys56.
Epitope
Acetylated Lys56
Host
Rabbit
Specificity
This antibody recognizes Histone H3 acetylated at Lys56.
Species Reactivity
Human
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_003520
Gene Symbol
HIST1H3A
H3FA
HIST1H3B
H3FL
HIST1H3C
H3FC
HIST1H3D
H3FB
HIST1H3E
H3FD
HIST1H3F
H3FI
HIST1H3G
H3FH
HIST1H3H
H3FK
HIST1H3I
H3FF
HIST1H3J
H3FJ
Purification Method
Affinity Purfied
UniProt Number
P68431
Molecular Weight
~17 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa acid extract, sodium butyrate treated HeLa acid extract, colcemid treated HeLa acid extract, recombinant Histone H3, recombinant Histone H4, and recombinant Histone H2A.
Western Blot Analysis: A 1:405 dilution of this antibody detected acetyl-Histone H3 (Lys56) in sodium butyrate treated HeLa acid extract.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
