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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Rb | IP, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-728 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-HRI Antibody |
| Product Information | |
|---|---|
| Format | Serum |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect HRI using this Anti-HRI Antibody validated for use in IP & WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | 6His-tagged fusion protein corres-ponding to residues 1-138 of mouse heme-regulated inhibitor (HRI) |
| Host | Rabbit |
| Specificity | HRI |
| Isotype | IgG |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The HRI gene is localized to 7p22 where its 3 end slightly overlaps the 3 end of the gene JTV1. The two genes are transcribed from opposite strands. Studies in rat and rabbit suggest that the HRI gene product phosphorylates the alpha subunit of eukaryotic initiation factor 2. Its kinase activity is induced by low levels of heme and inhibited by the presence of heme. |
| Gene Symbol |
|
| Purification Method | Serum |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q9BQI3 # Mediates down-regulation of protein synthesis in response to various stress conditions by the phosphorylation of EIF2S1 at Ser-48 and Ser-51. Protein synthesis is inhibited at the level of initiation (By similarity). SIZE: 630 amino acids; 71106 Da SUBUNIT: Synthesized in an inactive form that binds to the N- terminal domain of CDC37. Has to be associated with a multiprotein complex containing Hsp90, CDC37 and PPP5C for maturation and activation by autophosphorylation. The phosphatase PPP5C modulates this activation. Homodimer; non-covalently bound in the absence of hemin. Converted to an inactive disulfide linked homodimer in the presence of hemin (By similarity). TISSUE SPECIFICITY: Detected in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, muscle and stomach. PTM: Activated by autophosphorylation; phosphorylated predominantly on serine and threonine residues, but also on tyrosine residues (By similarity). SIMILARITY: SwissProt: Q9BQI3 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. & Contains 2 HRM (heme regulatory motif) repeats. & Contains 1 protein kinase domain. MISCELLANEOUS: Can bind 2 molecules of heme per polypeptide chain (By similarity). |
| Molecular Weight | 72kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot on lysates from mouse reticulocytes. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at -20°C from date of shipment |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |