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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Ca | WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-741 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-AS160 (Rab-GAP) Antibody |
| Alternate Names |
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| Background Information | AS160 (Akt Substrate of 160 kDa, Akt1S1), Rab GAP (GTPase activating protein), is phosphorylated on multiple sites by Akt in response to insulin in adipocytes and contraction in muscles. AS160 is the long sought after link between the upstream insulin signaling through insulin/PI3K/Akt and GLUT4 vessicle translocation to the cell membrane. It has 6 Akt phosphorylation consensus sequences that are believed to become phosphorylated upon insulin stimulation. This phosphorylation is required for translocation of GLUT4-containing vesicles to the cell membrane1. A mutation of two or more of these phosphorylation sites results in a dramatic decrease in AS160 activity and insulin-stimulated GLUT4 redistribution at the cell membrane2. AS160 has also shown to be important for GLUT4 expcytosis2. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit polyclonal antiserum IgG in buffer containing 0.05% sodium azide and 30% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect AS160 (Rab-GAP) using this Anti-AS160 (Rab-GAP) Antibody validated for use in WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated, synthetic peptide corresponding to amino acids 1178-1189 (C-PTNDKAKAGNKP) of rat Akt substrate of 160kDa (AS160) (Rab-GAP) with a N-terminal cysteine for conjugation purposes. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Recognizes AS160 (Rab-GAP), Mr 160 kDa. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human and mouse. Predicted to cross react with rat and dog based on sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: O60343 # May act as a GTPase-activating protein for Rab family protein(s). SIZE: 1298 amino acids; 146563 Da TISSUE SPECIFICITY: Expressed in heart, skeletal muscle and in immune tissues (spleen, lymph node and leukocytes). PTM: Phosphorylated by AKT1; insulin-induced. & Insulin-stimulated phosphorylation is required for GLUT4 translocation (By similarity). DISEASE: SwissProt: O60343 # May be involved in atopic dermatitis (AD). SIMILARITY: SwissProt: O60343 ## Contains 2 PID domains. & Contains 1 Rab-GAP TBC domain. MISCELLANEOUS: Subcellular redistribution from low density microsomes to the cytosol with insulin treatment (By similarity). |
| Molecular Weight | Approx. 160 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot in RIPA lysates from 3T3/L1 cells differentiated into adipocytes. Western Blot Analysis: A 1:1000-1:5000 dilution of this lot detected AS160 (Rab-GAP) in RIPA lysates from 3T3/L1 cells differentiated into adipocytes by MDI treatment. This lot also detects AS160 (Rab-GAP) in HEK293 lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |