| Description | |
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| Catalogue Number | 12-439 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Retinoblastoma Protein (aa 773-928) |
| Overview | Recombinant fusion protein containing the C-terminal fragment (residues 773-928) of human retinoblastoma protein (Rb) with an N-terminal His6-tag, expressed in E. coli. |
| Product Information | |
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| Presentation | 50 mm Tris-HCl, pH 7.5, 150 mm NaCl, and 500 mm imidazole |
| Quality Level | MQ100 |
| Applications | |
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| Application | Recombinant fusion protein containing the C-terminal fragment (residues 773-928) of human retinoblastoma protein (Rb) with an N-terminal His6-tag, expressed in E. coli. |
| Key Applications |
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| Biological Information | |
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| Concentration | 2.0 mg/mL |
| Entrez Gene Number |
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| Entrez Gene Summary | Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral. Spontaneous regression (cure) occurs in some cases. The retinoblastoma gene RB was the first tumor suppressor gene cloned, and is a negative regulator of the cell cycle through its ability to bind the transcription factor E2F (MIM 189971) and repress transcription of genes required for S phase (Hanahan and Weinberg, 2000 [PubMed 10647931]).[supplied by OMIM] |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P06400 # Key regulator of entry into cell division that acts as a tumor suppressor. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 Lys-20 trimethylation. Also acts as a transcription repressor of E2F target genes by recruiting chromatin-modifying enzymes to promoters. Inhibits the intrinsic kinase activity of TAF1. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. SIZE: 928 amino acids; 106159 Da SUBUNIT: Interacts preferentially with transcription factor E2F1. The unphosphorylated form interacts with ARID3B, JARID1A, SUV39H1, MJD2A/JHDM3A and THOC1. Interacts with the N-terminal domain of TAF1. Interacts with AATF, DNMT1, LIN9, LMNA, SUV420H1, SUV420H2, PELP1 and TMPO-alpha. May interact with KNTC2. Interacts with EID1 and ZUBR1. Interacts with ARID4A and JARID1B. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in the retina. PTM: Phosphorylated from S to M phase of the cell cycle and is dephosphorylated in G1. T, but not E1A, binds only to the unphosphorylated form. DISEASE: SwissProt: P06400 # Defects in RB1 are the cause of childhood cancer retinoblastoma (RB) [MIM:180200]. RB is a congenital malignant tumor that arises from the nuclear layers of the retina. It occurs in about 1:20000 live births and represents about 2% of childhood malignancies. It is bilateral in about 30% of cases. Although most RB appear sporadically, about 20% are transmitted as an autosomal dominant trait with incomplete penetrance. The diagnosis is usually made before the age of 2 years when strabismus or a gray to yellow reflex from pupil (cat eye) is investigated. & Defects in RB1 are a cause of bladder cancer [MIM:109800]. & Defects in RB1 are a cause of osteogenic sarcoma [MIM:259500]. SIMILARITY: SwissProt: P06400 ## Belongs to the retinoblastoma protein (RB) family. |
| Product Usage Statements | |
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| Quality Assurance | Routinely evaluated as a sustrate for many Serine/Threonine kinases. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. |
| Packaging Information | |
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| Material Size | 500 µg |