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Key Specifications Table
| Key Applications | Entrez Gene Number | Uni Prot Number |
|---|---|---|
| KA | NM_005321.2 | P10412 |
| Description | |
|---|---|
| Catalogue Number | 14-155 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Histone H1 Protein, 20 mg |
| Alternate Names |
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| Background Information | H1 and related linker histones are important both for maintenance of higher-order chromatin structure and for the regulation of gene expression. Nucleosomal stabilization is provided through the binding of histone H1 to the nucleosomal dyad and the linker DNA entering and exiting the core particle. Additional experimental evidence illustrates the influence of histone H1 on chromatin arrangement and compaction. The family of histone H1 proteins contains five somatic variants (H1.1, H1.2, H1.3, H1.4 and H1.5) which are expressed in nearly all cell types. |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Purified bovine Histone H1 protein for use as a substrate in histone modification assays (HAT, HDAC, DNMT) and chromatin assembly studies. |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | Lysine rich fraction from calf thymus |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. |
| Gene Symbol |
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| Purification Method | Sterilized through a 0.2 micron membrane filter |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P10412 # Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. SIZE: 219 amino acids; 21865 Da SUBCELLULAR LOCATION: Nucleus. SIMILARITY: SwissProt: P10412 ## Belongs to the histone H1/H5 family. MISCELLANEOUS: This variant accounts for 60% of histone H1. |
| Molecular Weight | 32kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Kinase assay using cdk2/cyclin A, active (14-448) to phosphorylate Histone H1. |
| Sales Restrictions | This product is derived from bovine source. Export of this product to certain countries may be restricted. Please contact Customer Service or your local distributor to inquire about product availability and export options. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 1 year at -20°C |
| Packaging Information | |
|---|---|
| Material Size | 20 mg |