Key Specifications Table
| Key Applications | Entrez Gene Number | Uni Prot Number |
|---|---|---|
| HAT | NM_003884.3 | Q92831 |
| Description | |
|---|---|
| Catalogue Number | 14-309 |
| Brand Family | Upstate |
| Trade Name |
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| Description | PCAF Protein, Active, 50 µg |
| Overview | Recombinant GST fusion protein |
| Product Information | |
|---|---|
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | PCAF recombinant GST fusion protein, for use in Histone Acetyltransferase Assays. (HAT). |
| Key Applications |
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| Biological Information | |
|---|---|
| Source | expressed in E. Coli corresponding to amino acids 352-832 |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q92831 # Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tats transactivating activity and may help inducing chromatin remodeling of proviral genes. SIZE: 832 amino acids; 93013 Da SUBUNIT: Interacts with SIRT1 (By similarity). Interacts with EP300 and CREBBP. Interacts with NCOA1 and NCOA3. Binds to HTLV-1 Tax. Interacts with and acetylates HIV-1 Tat. SUBCELLULAR LOCATION: Nucleus (By similarity). TISSUE SPECIFICITY: Ubiquitously expressed but most abundant in heart and skeletal muscle. DOMAIN:SwissProt: Q92831 The bromodomain mediates binding to HIV-1 Tat. SIMILARITY: Belongs to the GCN5 family. & Contains 1 bromo domain. & Contains 1 N-acetyltransferase domain. |
| Molecular Weight | 79kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | routinely evaluated by acetylation of core histones in vitro |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 6 months at -20°C |
| Packaging Information | |
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| Material Size | 50 µg |