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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IP, WB, IH(P) | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1033 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Versican Antibody, a.a. 1360-1439 of mouse versican |
| Alternate Names |
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| Background Information | Versican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan., Versican has an apparent molecular mass of ca. 1000 kDa, of which the core contributes 400 kDa from the 2409 amino acids, and the 12 -15 covalently attached chondroitin sulphate chains make up the rest. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | The immunoglobulin was affinity purified against the recombinant antigen and is presented in PBS. Anti-GST reactivity has not been adsorbed. Liquid in PBS. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Versican Antibody, a.a. 1360-1439 of mouse versican is an antibody against Versican for use in IP, WB, IH(P). |
| Key Applications |
|
| Application Notes | Immunohistochemistry (formally fixed, paraffin embedded): 10 μg/mL. Tissues must be treated with Chondroitinase before staining. {0.5U/mL in 100mM Tris-HCL pH 7.2-7.4, 30 minutes at room temperature} Then block and stain. Western blotting: 0.5 μg/mL Immunoprecipitation: Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | GST fusion protein containing amino acids 1360-1439 of mouse versican. |
| Epitope | a.a. 1360-1439 of mouse versican |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Mouse versican GAG beta domain. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. |
| Gene Symbol |
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| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P16112 # This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. SIZE: 2415 amino acids; 250193 Da SUBUNIT: Interacts with FBLN1 (By similarity). SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity). TISSUE SPECIFICITY: Restricted to cartilages. DEVELOPMENTAL STAGE: Expression was detected in chondrocytes throughout the developing skeleton. DOMAIN: SwissProt: P16112 Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3. PTM: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase. DISEASE: SwissProt: P16112 # Defects in AGC1 are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK) [MIM:608361]. Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy. SIMILARITY: Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 1 EGF-like domain. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 4 Link domains. & Contains 1 Sushi (CCP/SCR) domain. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeat freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |