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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Rhesus Macaque | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB10541 |
| Description | Anti-Galectin-3 Antibody |
| Alternate Names |
|
| Background Information | Galectin-3 belongs to the galectin gene family, which is defined by the specificity of a carbohydrate recognition domain (CRD) for Gal/Lac. Galectin-3 expression is usually elevated in a wide range of neoplastic cell types. The galectin CRD consists of 135 amino acids. Galectin-3 is known to play a role in tumor development, apoptosis, and cell cycle progression. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Galectin-3 Antibody is validated for use in WB for the detection of Galectin-3. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the Pro64 mutation domain of human Galectin-3. |
| Epitope | Pro64 mutation domain |
| Host | Rabbit |
| Specificity | This antibody recognizes the Pro64 mutation domain of Galectin-3. It is equally specific for wild-type or mutant His64 Galectin-3. |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Predicted to react with Rhesus Macaque based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants. |
| Gene Symbol |
|
| Purification Method | Affinity Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. SUBUNIT STRUCTURE: Probably forms homo- or heterodimers. Interacts with DMBT. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Secreted. Note: Secreted by a non-classical secretory pathway and associates with the cell surface. TISSUE SPECIFICITY: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. |
| Molecular Weight | ~33 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in MG-63 cell lysate. Western Blot Analysis: A 1:250 dilution of this antibody detected Galectin-3 in 10 µg of MG-63 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |