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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | IHC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB120 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Norepinephrine Antibody |
| Alternate Names |
|
| Background Information | The chemical compound norepinephrine (NC(8)O(3)H(11), also known as noradrenaline, is a catecholamine that functions as both a neurotransmitter and a hormone. It is released from the adrenal glands and also by certain neurons. It affects parts of the human brain where attention and impulsivity are controlled. This compound affects the fight-or-flight response, activating the sympathetic nervous system to directly increase heart rate, release energy from fat and increase muscle readiness. The host of physiological changes activated by a stressful event are unleashed in part by activation of a nucleus in the brain stem called the locus ceruleus. This nucleus is the origin of most norepinephrine pathways in the brain. Neurons using norepinephrine as their neurotransmitter project bilaterally from the locus ceruleus along distinct pathways to the cerebral cortex, limbic system, and the spinal cord, among other projections. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Liquid rabbit antiserum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Norepinephrine Antibody is an antibody against Norepinephrine for use in IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:200-1:500 with enhanced detection. Glutaraldehyde fixation required for reactivity. Lightly fixed 4% PFA .2-5% glutaraldehyde fixed tissue works well; permeabilization with 0.1-.2% triton X-100 in block ONLY. Dilute antibody solutions in simple buffers without excess proteins or detergents. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Noradrenaline-glutaraldehyde-BSA |
| Host | Rabbit |
| Specificity | Noradrenaline: reactive with noradrenaline reacted with glutaraldehyde; glutaraldehyde required in fixation for reactivity; reactivity with free noradrenaline is very poor. The cross-reactivities were determined using either ELISA or RIA techniques, at concentration/unconjugated or conjugated amino acid concentration at half displacement. Compound Cross-reactivity Noradrenaline-G-BSA 1.0 Octopamine-G-BSA 0.032 Dopamine-G-BSA 0.016 Adrenaline-G-BSA 0.006 Noradrenaline 0.0002 L-Dopa-G-BSA 0.0002 p-Tryamine-G-BSA 0.0002 *The antisera was also tested for specificity using the free-floating PAP technique on rat locus coeruleus. Abbreviations: (BSA)= Bovine serum albumin G)= Glutaraldehyde |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |