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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | IHC, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1252 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Cytochrome P450 Enzyme CYP2E1 Antibody |
| Alternate Names |
|
| Background Information | Cytochrome P450 (CYP) is a huge and diverse superfamily of hemoproteins. It metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. Most CYPs can metabolize multiple substrates, and can catalyze multiple reactions. This accounts for their central importance in metabolizing the extremely large number of endogenous and exogenous molecules. In the liver, these substrates include drugs and toxic compounds as well as metabolic products such as bilirubin (a breakdown product of hemoglobin). Cytochrome P450 enzymes are present in many other tissues of the body including the mucosa of the gastrointestinal tract, and play important roles in hormone synthesis and breakdown (including estrogen and testosterone synthesis and metabolism), cholesterol synthesis, and vitamin D metabolism. More than 7700 distinct CYP sequences are known as of September 2007. |
| Product Information | |
|---|---|
| Format | Serum |
| Control |
|
| Presentation | Rabbit polyclonal antiserum in buffer containing no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Cytochrome P450 Enzyme CYP2E1 Antibody is an antibody against Cytochrome P450 Enzyme CYP2E1 for use in IH & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: A previous lot was used on formaldehyde treated and frozen sections. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide. |
| Host | Rabbit |
| Specificity | Reacts with human and rat cytochrome P450 enzyme CYP2E1 in hepatic microsomal fractions. No cross-reactivity with other cytochrome P450 enzymes in these species. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. |
| Gene Symbol |
|
| Purification Method | Unpurified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P05181 # Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. SIZE: 493 amino acids; 56849 Da SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. SIMILARITY: SwissProt: P05181 ## Belongs to the cytochrome P450 family. |
| Molecular Weight | 57 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on Human brain lysates. Western Blot Analysis: 1:1000 dilution of this antibody detected Cytochrome P450 CYP2E1 on 10 μg of Human brain lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |