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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | WB, IH(P), PIA | Ch | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1286-I |
| Replaces | AB1286 |
| Description | Anti-Monocarboxylate Transporter 1 Antibody |
| Alternate Names |
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| Background Information | Monocarboxylate transporter 1 (UniProt: P53987; also known as MCT 1, Solute carrier family 16 member 1) is encoded by the Slc16a1 (also known as Mct1) gene (Gene ID: 25027) in rat. MCT1 is a multi-pass membrane protein that is detected in erythrocytes, astrocytes, heart, kidney, muscle, and small intestine. It contains 12 transmembrane domains, 6 extracellular domains, and 7 cytoplasmic domains. It serves a proton-coupled monocarboxylate transporter that catalyzes the rapid transport across the plasma membrane of many monocarboxylates, including lactate, pyruvate, branched-chain oxo acids, acetoacetate, and beta-hydroxybutyrate. Depending on the tissue and on circumstances, it mediates the import or export of lactic acid and ketone bodies. It is shown to be essential for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. It also plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate with concomitant effects on plasma insulin levels and blood glucose homeostasis. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified chicken polyclonal IgY in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Monocarboxylate Transporter 1, Cat. No. AB1286-I, is a chicken polyclonal antibody that detects Monocarboxylate Transporter 1 and is tested in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (Paraffin) Analysis: A 1:1,000-2,500 dilution from a representative lot detected Monocarboxylate Transporter 1 in rat kidney and rat skeletal muscle tissue sections. Peptide Inhibition Assay: Target band detection in rat brain membrane lysate was prevented by pre-blocking of a representative lot with the immunogen peptide. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the C-terminus of rat Monocarboxylate Transporter 1. |
| Epitope | C-terminus |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Chicken |
| Specificity | This chicken polyclonal antibody detect Monocarboxylate Transporter 1. It targets an epitope within 15 amino acids from the C-terminal region. |
| Isotype | IgY |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Rat. Predicted to react with Mouse based on immunogen design. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | ~53 kDa observed; 53.24 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Rat brain membrane lysate. Western Blotting Analysis: 0.25 µg/mL of this antibody detected Monocarboxylate Transporter 1 in Rat brain membrane lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |