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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, H, Ch, Av, Gp, Op | ICC, IHC, WB | Gt | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB144P-200UL |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Choline Acetyltransferase Antibody |
| Alternate Names |
|
| Background Information | Acetylcholine (ACh) is a common neuro-transmitter for motoneurons, preganglionic autonomic neurons, postganglionic para-sympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified goat polyclonal in buffer containing PBS containing 5 mg/mL BSA and 0.2% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Choline Acetyltransferase (ChAT) Antibody is validated for use in IC, IH, WB for the detection of Choline Acetyltransferase. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: A previous lot of this antibody was used in immunohistochemistry at 1:100 dilution. See attached protocol. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Human placental enzyme. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Goat |
| Specificity | Choline acetyltransferase found in cholinergic neurons in brain and central nervous systems. |
| Species Reactivity |
|
| Species Reactivity Note | Rat, mouse, monkey, guinea pig, chicken, opossum, avian and human (western blot). |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq]. Transcript Variant: This variant (M) contains alternate 5 exon M, uses an upstream start codon, and encodes isoform 2. |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. SIZE: 748 amino acids; 82568 Da DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive. SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family. |
| Molecular Weight | 70 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot on NIH/3T3 lysates. Western Blot Analysis: 1:1000 dilution of this antibody detected ChAT on 10 µg of NIH/3T3 lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C in undiluted aliquots from date of receipt. For short term storage (3-4 weeks) maintain at +2-8°C. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 200 µL |