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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ELISA, WB, NEUT | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB1498P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Stem Cell Factor Antibody |
| Alternate Names |
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| Background Information | Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble form is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Affinity purified immunoglobulin. Lyophilized from 0.5X PBS pH 7.2. Reconstitute with sterile distilled water. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Stem Cell Factor using this Anti-Stem Cell Factor Antibody validated for use in ELISA, WB, NEUT. |
| Key Applications |
|
| Application Notes | ELISA: To detect mSCF by direct ELISA (using 100 μL/well antibody solution) a concentration of at lease 0.5 μg/mL of this antibody is required. Should allow detection of 0.2 - 0.4 ng/well of recombinant mSCF. Neutralization: For ½ maximal inhibition (ND50) of the bioogical activity of mSCF (20 ng/mL), a concentration of 0.64 - 0.97 μg/mL is required. Western blot: 0.1 - 0.2 μg/mL, this dilution should offer a detection limit of 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Highly pure (>98%) recombinant mouse SCF |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Reacts with mouse SCF; SCF is highly conserved cross reactivity with rat, human and other species SCF is expected but untested. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P21583 # Stimulates the proliferation of mast cells. Able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. Mediates also cell-cell adhesion. Acts synergistically with other cytokines, probably interleukins. SIZE: 273 amino acids; 30899 Da SUBUNIT: Homodimer, non-covalently linked (Probable). SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein (By similarity). Note=Also exists as a secreted soluble form (isoform 1 only) (By similarity). & Isoform 2: Cell membrane; Single-pass type I membrane protein (By similarity). DEVELOPMENTAL STAGE: Acts in the early stages of hematopoiesis. PTM: A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain. SIMILARITY: SwissProt: P21583 ## Belongs to the SCF family. |
| Stem Cell Type |
|
| Molecular Weight | 30 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile water to a concentration of = 0.2 mg/ml the antibody is stable for at least six weeks at 2-4ºC. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |