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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | IHC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB15348 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-FMRFamide Antibody |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Liquid with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-FMRFamide Antibody is an antibody against FMRFamide for use in IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:1,000 - 1:5,000 Application Notes for Immunohistochemistry: - Suggested perfusion steps: 1) Ca2+-free Tyrodes solution 2) Paraformaldehyde-picric acid fixative 3) 10% sucrose in PBS as a cryo-protectant - Rapidly dissect tissue and store overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. - Incubate slide-mounted tissue sections with blocking buffer for 1 hour at room temperature. - Remove blocking buffer and incubate the slides with the primary antibody (AB15348 diluted to desired working concentration in blocking buffer) for 18-24 hours at 2-8°C. - Remove primary antibody and rinse 3 times in PBS. - Incubate sections with fluorescently labeled secondary antibody for 60 minutes at room temperature. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | FMRFamide |
| Host | Rabbit |
| Specificity | FMRFamide |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | FMFRamide-related protein precursor plays a role in the regulation of heart rate and blood pressure and the modulation of morphine-induced antinociception. FMRFAL encodes a preproprotein which is cleaved to form two active peptides with similar function. |
| Gene Symbol |
|
| Purification Method | Serum |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: O15130 # Morphine modulating peptides. Have wide-ranging physiologic effects, including the modulation of morphine-induced analgesia, elevation of arterial blood pressure, and increased somatostatin secretion from the pancreas. Neuropeptide FF potentiates and sensitizes ACCN2 and ACCN3 channels. SIZE: 113 amino acids; 12440 Da SUBCELLULAR LOCATION: Secreted. SIMILARITY: SwissProt: O15130 ## Belongs to the FARP (FMRFamide related peptide) family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |